Laboratory of Subcellular Structures Analyses

Prof. Eliza Wyszko

Head of Laboratory

eliza.wyszko@ibch.poznan.pl
ext. 1141

Research Projects

Keywords

low molecular compounds,
nucleosides,
DNA modifications,
catalytic nucleic acids,
gene expression,
confocal microscopy,
flow cytometry,
real time PCR,
xCELLigence,
CO2 incubator,
laminar flow cabinet,
high-performance liquid chromatography,
HPLC,
MS

Offer

Confocal microscopy:

Visualization of intravital and fixed preparations,
Analysis of fluorochromes, ex. 470-670 nm and 405 nm,
2D and 3D analysis of images,
FRET, FRAP,
Analysis of fluorescence intensity,
Colocalization,

Flow cytometry:

Analysis of cell cycle,
Analysis of cell toxicity (apoptosis, necrosis),
Immunofluorescent staining,
Analysis of oxidative stress,
Cell sorting,

System xCELLigence:

Real time analysis of cell proliferation or migration,
Termocycler LightCycler 480,
Real time PCR with fluorescent probes,
HRM analysis of point mutations,
Analysis of methylation level and genotyping,

High-performance liquid chromatography (HPLC):

Analysis of nucleic acid modifications in eukaryotic cells.
Possibility of carrying out the entire experimental process including cell culturing and the use of specialized equipment by PASS.

Equipment

Confocal microscope Leica TCS SP5 II with white laser and intravital chamber with LAS AF SP5 software and LAS X SP8 software with deconvolution
DMI 4000B Leica microscope: fluorescence, bright field
Stereoscopic microscope Leica
High-performance liquid chromatography (HPLC) with detectors

■

UV,
■

fluorescent (FLD),
■

electrochemical (ED) and mass (MSI)

FACSCalibur flow cytometer (Becton Dickinson)
Accuri C6 flow cytometer (Becton Dickinson)
Cell harvester (96 wells) Perkin Elmer
Real time PCR LightCycler 480 II Roche
xCELLignce system
Cell culture CO2 incubator INCO 153 Memmert
Laminar flow cabinet BioBan 48 for sterile cell culturing

Research activity

Anti-aging properties of 4-N-furfyrylocytosine in age-differentiated eukaryotic cells, budding yeast and mouse model of aging. NSC OPUS (EW)
Kinetin riboside and its derivatives - analysis of apoptotic properties and mechanism of action in brain tumor cells. NSC OPUS (EW)
Analysis of pro-regenerative and antioxidant properties of 4-N-furfurylcytosine in an animal model of Schmidtea mediterranea (Planaria). NSC MINIATURA (AFW)
Optimization biodegradation conditions to create a 3D cellular model of liver cancer as a screening platform for the selection of compounds with therapeutic potential. NSC MINIATURA (DGW)
Cooperation:
The regulation of neuroactive beta-carboline levels in diet based on the coffee substitute. NSC OPUS (Prof. Renata Zawirska-Wojtasiak, PULS)
Molecules binding DNA – synthesis and properties of DNA intercalators containing boran clusters. SONATA BIS (Dr hab. Agnieszka Olejniczak, IBM PAS)
pH-sensitive oligonucleotides for monitoring changes in cancer cells metabolism induced by anticancer drugs. NCRD TANGO (Dr hab. Anna Dembska, AMU)
RNA hybrid duplex-quadruplex structures with dual functionality as a new tool for silencing of gene expression. NSC OPUS (Prof. Zofia Gdaniec, IBCH PAS)

Selected publications

Rykowski S, Gurda-Woźna D, Fedoruk-Wyszomirska A, Orlicka-Płocka M, Kowalczyk A, Stączek P, Denel-Bobrowska M, Biniek-Antosiak K, Rypniewski W, Wyszko E, Olejniczak AB. Carboranyl-1,8-naphthalimide intercalators induce lysosomal membrane permeabilization and ferroptosis in cancer cell lines. J Enzyme Inhib Med Chem. 2023, 38:2171028. doi: 10.1080/14756366.2023.2171028
Barciszewska AM, Belter A, Gawrońska I, Giel-Pietraszuk M, Naskręt-Barciszewska MZ. Juglone in Combination with Temozolomide Shows a Promising Epigenetic Therapeutic Effect on the Glioblastoma Cell Line. Int J Mol Sci. 2023, 24:6998. doi: 10.3390/ijms24086998
Pawelczak P, Fedoruk-Wyszomirska A, Wyszko E. Antiaging Effect of 4-N-Furfurylcytosine in Yeast Model Manifests through Enhancement of Mitochondrial Activity and ROS Reduction. Antioxidants (Basel). 2022, 11:850. doi: 10.3390/antiox11050850.
Rykowski S, Gurda-Woźna D, Orlicka-Płocka M, Fedoruk-Wyszomirska A, Giel-Pietraszuk M, Wyszko E, Kowalczyk A, Stączek P, Biniek-Antosiak K, Rypniewski W, Olejniczak AB. Design of DNA Intercalators Based on 4-Carboranyl-1,8-Naphthalimides: Investigation of Their DNA-Binding Ability and Anticancer Activity. Int J Mol Sci. 2022, 23:4598. doi: 10.3390/ijms23094598
Barciszewska AM, Belter A, Gawrońska I, Giel-Pietraszuk M, Naskręt-Barciszewska MZ. Cross-reactivity between histone demethylase inhibitor valproic acid and DNA methylation in glioblastoma cell lines. Front Oncol. 2022, 12:1033035. doi: 10.3389/fonc.2022.1033035
Wyszko E, Popenda M, Gudanis D, Sarzyńska J, Belter A, Perrigue P, Skowronek P, Rolle K, Barciszewski J. The model structure of the hammerhead ribozyme formed by RNAs of reciprocal chirality. Biosci Rep. 2021, 41:BSR20203424. doi: 10.1042/BSR20203424
Orlicka-Płocka M, Fedoruk-Wyszomirska A, Gurda-Woźna D, Pawelczak P, Krawczyk P, Giel-Pietraszuk M, Framski G, Ostrowski T, Wyszko E. Implications of Oxidative Stress in Glioblastoma Multiforme Following Treatment with Purine Derivatives. Antioxidants (Basel). 2021, 10:950. doi: 10.3390/antiox10060950.
Rykowski S, Gurda-Woźna D, Orlicka-Płocka M, Fedoruk-Wyszomirska A, Giel-Pietraszuk M, Wyszko E, Kowalczyk A, Stączek P, Bak A, Kiliszek A, Rypniewski W,Olejniczak AB. Design, Synthesis, and Evaluation of Novel 3-Carboranyl-1,8-Naphthalimide Derivatives as Potential Anticancer Agents. Int J Mol Sci. 2021, 22:2772. doi: 10.3390/ijms22052772
Wawrzyniak D, Grabowska M, Głodowicz P, Kuczyński K, Kuczyńska B, Fedoruk-Wyszomirska A, Rolle K. Down-regulation of tenascin-C inhibits breast cancer cells development by cell growth, migration, and adhesion impairment. PLoS One. 2020 Aug 20;15(8):e0237889. doi: 10.1371/journal.pone.0237889
Zawirska-Wojtasiak R, Fedoruk-Wyszomirska A, Piechowska P, Mildner-Szkudlarz S, Bajerska J, Wojtowicz E, Przygoński K, Gurda D, Kubicka W, Wyszko E. β-Carbolines in Experiments on Laboratory Animals. Int J Mol Sci. 2020, 21:5245. doi: 10.3390/ijms21155245
Orlicka-Płocka M, Gurda-Wozna D, Fedoruk-Wyszomirska A, Wyszko E. Circumventing the Crabtree effect: forcing oxidative phosphorylation (OXPHOS) via galactose medium increases sensitivity of HepG2 cells to the purine derivative kinetin riboside. Apoptosis. 2020, 25:835-852. doi: 10.1007/s10495-020-01637-x.
Różycka D, Korycka-Machała M, Żaczek A, Dziadek J, Gurda D, Orlicka-Płocka M, Wyszko E, Biniek-Antosiak K, Rypniewski W, Olejniczak AB. Novel Isoniazid-Carborane Hybrids Active in Vitro Against Mycobacterium tuberculosis. Pharmaceuticals (Basel). 2020, 13:465. doi: 10.3390/ph13120465
Dembska A, Świtalska A, Fedoruk-Wyszomirska A, Juskowiak B. Development of fluorescence oligonucleotide probes based on cytosine- and guanine-rich sequences. Sci Rep. 2020, 10:11006. doi: 10.1038/s41598-020-67745-5.
Nekvinda J, Różycka D, Rykowski S, Wyszko E, Fedoruk-Wyszomirska A, Gurda D, Orlicka-Płocka M, Giel-Pietraszuk M, Kiliszek A, Rypniewski W, Bachorz R, Wojcieszak J, Grüner B, Olejniczak AB. Synthesis of naphthalimide-carborane and metallacarborane conjugates: Anticancer activity, DNA binding ability. Bioorg Chem. 2020, 94:103432. doi: 10.1016/j.bioorg.2019.103432.
Niazi AK, Delannoy E, Iqbal RK, Mileshina D, Val R, Gabryelska M, Wyszko E, Soubigou-Taconnat L, Szymanski M, Barciszewski J, Weber-Lotfi F, Gualberto JM, Dietrich A. Mitochondrial Transcriptome Control and Intercompartment Cross-Talk During Plant Development. Cells. 2019, 8:583. doi:10.3390/cells8060583.

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Laboratory of Subcellular Structures Analyses

Researchers:

Research and technical employees

PhD students:

Research Projects

Keywords

Offer

Equipment

Research activity

Selected publications

Adres

Address

Laboratory of Subcellular Structures Analyses

Researchers:

Research and technical employees

PhD students:

Research Projects

Keywords

Rys. 1

Rys. 2.

Rys. 3.

Rys.4.

Rys. 5.

Offer

Equipment

Research activity

Selected publications

Adres

Address